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EMARS朄偺奐敪偲墳梡
EMARS朄偺奐敪丗
EMARS朄偼丄惗偒偰偄傞嵶朎偺嵶朎昞柺偱夛崌偡傞暘巕傪尒偮偗傞偨傔偺曽朄偱偁傞乮1-3乯丅変乆偼嬼慠偵丄捠忢岝傾僼傿僯僥傿乕儔儀儕儞僌偵棙梡偝傟傞傾儕乕儖傾僕僪婎偑惣梞儚僒價儁儖僆僉僔僟乕僛乮HRP乯偵傛偭偰僫僀僩儗儞儔僕僇儖傪惗偠傞偙偲傪敪尒偟丄enzyme-mediated activation of radical sources乮EMARS乯偲柦柤偟偨乮1乯丅
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EMARS斀墳
嶻惗偝傟偨儔僕僇儖偼抁柦偱徚戅偡傞傑偱偵尷傜傟偨嫍棧偟偐撏偐側偄偺偱丄僾儘乕僽暘巕偺嬤朤暘巕偵偺傒峌寕偟嫟桳寢崌偡傞丅柶塽揹尠偵傛傝丄EMARS 斀墳偵傛傝僾儘乕僽暘巕偺廃埻200乣300 nm 偺斖埻偺暘巕偑昗幆偝傟傞偙偲傪妋擣偟偨乮1乯丅偙偺嫍棧偼巋寖傪庴偗偰婑傝廤傑偭偨帀幙儔僼僩偺僒僀僘偵堦抳偡傞丅
EMARS斀墳偱昗幆偝傟偨暘巕偼丄峈懱傾儗僀僔僗僥儉偱摨掕偟偨乮1-3乯丅峈懱傾儗僀僔僗僥儉偼崅姶搙偵丄偐偮丄梕堈偵暘巕傪摨掕偱偒傞偑丄尷傜傟偨庬椶偺峈懱偟偐搵嵹偝傟偰偄側偄丅偙偺晄懌傪曗姰偡傞偨傔偵丄EMARS嶻暔偺摨掕偵幙検暘愅偵傛傞僾儘僥僆儈僋僗暘愅朄傪梡偄偨乮4乯丅
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峈懱傾儗僀偵傛傞EMARS昗幆僞儞僷僋偺摨掕
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幙検暘愅偵傛傞EMARS昗幆僞儞僷僋偺摨掕
EMARS朄偺夵椙丗
昗幆帋栻偵僼儖僆儗僙僀儞僠儔儈僪傪梡偄傞夵椙EMARS朄傪奐敪偟偨乮10乯丅僠儔儈僪偺儔僕僇儖壔偵偼夁巁壔悈慺傪昁梫偲偡傞偑丄廬棃梡偄偰偄偨傾儕乕儖傾僕僪偲斾偟偰斀墳惈偑崅偔丄撪嵼惈峺慺偵傛傞旕摿堎揑斀墳傕梷惂偝傟偨丅偙偺夵慞偵傛傝丄嵶朎撪僆儖僈僱儔撪偺夛崌暘巕偺夝愅偑壜擻偲側偭偨乮10乯丅
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僠儔儈僪傪梡偄傞夵椙EMARS朄
EMARS朄偺墳梡丗
婡擻揑暘巕娫憡屳嶌梡傪尒偮偗傞偨傔偵EMARS朄傪妶梡偟丄僼傿僽儘僱僋僠儞埶懚嵶朎堏摦偵塭嬁傪媦傏偡兝1 僀儞僥僌儕儞偲ErbB4 娫偵偍偗傞嬻娫帪娫埶懚揑憡屳嶌梡乮5乯偲丄峈懱堛栻昳偺儕僣僉僔儅僽偺巋寖偵傛偭偰桿摫偝傟傞CD20偲FGFR3偺憡屳嶌梡乮6乯傪尒偄偩偟偨丅偝傜偵丄B嵶朎儕儞僷庮偵偍偄偰GPI-傾儞僇乕僞儞僷僋幙偺堦庬偺Thy-1偑摿掕偺僠儘僔儞僉僫乕僛宆庴梕懱偲憡屳嶌梡偡傞偙偲傪尒偄偩偟偨乮7乯丅
堚揱巕岺妛偱敪尰偝偣偨HRP傪梡偄偰EMARS斀墳傪峴偆怴僶乕僕儑儞偺EMARS僔僗僥儉傪庽棫偟偨(8-10)丅HRP傪帀幙儔僼僩撪偵敪尰偝偣傞偨傔丄HRP傪GPI-傾儞僇乕宆偵偟偨丅僸僩曵夡懀恑場巕乮DAF乯偲僸僩Thy-1 桼棃偺GPI 晅壛僔僌僫儖攝楍傪偦傟偧傟暿屄偵HRP偺C枛抂偵楢寢偟偨2 庬椶偺GPI-傾儞僇乕宆HRP梈崌僞儞僷僋幙乮HRP-GPI乯傪僸僩HeLa S3嵶朎偵敪尰偝偣丄惗偒偰偄傞嵶朎忋偱偙傟傜偺HRP-GPI 傪梡偄偰EMARS斀墳傪峴偭偨丅偦偺寢壥丄堎側傞GPI 晅壛僔僌僫儖攝楍傪傕偮HRP-GPI偼丄堎側傞N宆摐嵔晅壛傪庴偗丄堎側傞暘巕夛崌懱傪宍惉偟偨丅偝傜偵丄尦棃偺DAF偼HRP-DAFGPI偲丄尦棃偺Thy-1偼HRP-Thy1GPI偲傕偭傁傜夛崌偟丄DAF偼HRP-DAFGPI偲摨條偵僐儞僾儗僢僋僗宆摐嵔傪丄Thy-1偼HRP-Thy1GPI偲摨條偵僴僀儅儞僲乕僗宆摐嵔傪桳偟偰偄偨乮10乯丅埲忋偺偙偲偐傜丄奺GPI-傾儞僇乕僞儞僷僋暘巕庬偼GPI晅壛僔僌僫儖偵埶懚偟偰屌桳偺帀幙儔僼僩傪宍惉偡傞偙偲偑柧傜偐偲側偭偨丅偙傟傜偺幚尡寢壥偼丄EMARS朄偼屄乆偺帀幙儔僼僩僪儊僀儞傪暘暿偱偒傞偙偲傪帵偡丅
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GPI晅壛僔僌僫儖偵埶懚偟偨屌桳偺帀幙儔僼僩宍惉
敪昞榑暥
侾乯 Kotani N, Gu J, Isaji T, Udaka K, Taniguchi N, Honke K.: Biochemical visualization of cell surface molecular clustering in living cells. Proc. Natl. Acad. Sci. U. S. A. 2008;105:7405-7409
俀乯 Honke K, Kotani N.: The enzyme-mediated activation of radical source reaction: a new approach to identify partners of a given molecule in membrane microdomains. J. Neurochem. 2011; 116:690-695
俁乯Honke K, Kotani N.: Identification of cell-surface molecular interactions under living conditions by using the enzyme-mediated activation of radical sources (EMARS) method. Sensors 2012; 12:16037-16045
係乯Jiang S, Kotani N, Ohnishi T, Miyagawa-Yamguchi A, Tsuda M, Yamashita R, Ishiura Y, Honke K.: A proteomics approach to the cell-surface interactome using the enzyme-mediated activation of radical sources
reaction. Proteomics 2012; 12:54-62
俆乯Yamashita R, Kotani N, Ishiura Y, Higashiyama S, Honke K.: Spatiotemporally-regulated interaction between 兝1 integrin and ErbB4 that is involved in fibronectin-dependent cell migration. J. Biochem. 2011; 149:347-355
俇乯Kotani N, Ishiura Y, Yamashita R, Ohnishi T, Honke K.: Fibroblast growth factor receptor 3 (FGFR3) associated with the CD20 antigen regulates the rituximab-induced proliferation inhibition in B-cell lymphoma cells. J. Biol. Chem. 2012; 287:37109-37118
俈乯Ishiura Y, Kotani N, Yamashita R, Yamamoto H, Kozutsumi Y, Honke K.: Anomalous expression of Thy1 (CD90) in B-cell lymphoma cells and proliferation inhibition by anti-Thy1 antibody treatment. Biochem. Biophys. Res. Commun. 2010; 396:329-334
俉乯Miyagawa-Yamaguchi A, Kotani N, Honke K.: Expressed glycosylphosphatidylinositol-anchored horseradish peroxidase identifies co-clustering molecules in individual lipid raft domains PLoS ONE 2014; 9:e93054
俋乯Miyagawa-Yamaguchi A, Kotani N, Honke K.: Segregation of lipid rafts revealed by the EMARS method using GPI-anchored HRP fusion proteins. Trends. Glycosci. Glycotech. 2014; 26:59-69
侾侽乯Miyagawa-Yamaguchi A, Kotani N, Honke K.: Each GPI-anchored protein species forms a specific lipid raft depending on its GPI attachment signal. Glycoconj. J. 2015 Oct;32(7):531-40.
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